Part:BBa_K3912999
AP
AP and NA makes a molecular switch together to reflect whether the tau protein exists in the reaction system. The reaction that takes place is Recombinase Polymerase Amplification(RPA), in which we use two primers, called Amplification F and Amplification R. AP is the single-strand DNA that pairs with NA strand, with the tau-aptamer at the 3’ terminal of it, but it does not have any chemical bonds with the aptamer. There is a loop on it, which contains the sequence of Amplification F called Amp-F, and does not pair with the NA strand. In the RPA reaction, when tau protein is absent, no reaction will start because of the block on the aptamer; but when tau is present and pull away the aptamer from NA strand, AP will act as the primer to make a double-strand DNA molecule with NA strand as the template. And then the primer-binding sequence (also the probe-binding sequence) will exist on the prolonged AP strand for Amplification R to make a prolonged NA strand, with the binding sequence for Amplification F on it, thus the complete double-strand DNA molecule is formed and the RPA reaction will continue.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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